Device

Part:BBa_K2326006:Design

Designed by: Zhongxiu Hu   Group: iGEM17_BNDS_China   (2017-10-24)


LacI - pTac - gadA - GFP - Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1720
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1443
    Illegal AgeI site found at 1522
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3469


Design Notes

The translation rate, 300, is based on the translation rate of the local RBS of gadR in Lactobacillus brevis NCL912.


Source

RBS Calculator - Salis Lab

References